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1.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 136-141, 2022.
Article in Chinese | WPRIM | ID: wpr-936186

ABSTRACT

Objective: To explore the types and clinical characteristics of chronic rhinosinusitis with nasal polyps (CRSwNP) based on artificial intelligence and whole-slide imaging (WSI), and to explore the consistency of the diagnostic criteria of the Japanese epidemiological survey of refractory eosinophilic chronic rhinosinusitis (JESREC) in Chinese CRSwNP patients. Methods: The data of 136 patients with CRSwNP (101 males and 35 females, aging 14 to 70 years) who underwent endoscopic sinus surgery from 2018 to 2019 in the Department of Otorhinolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University were analysed retrospectively. The preoperative clinical characteristics of patients were collected, such as visual analogue scale (VAS) of nasal symptoms, peripheral blood inflammatory cell count, total immunoglobulin E (IgE), Lund-Kennedy score and Lund-Mackay score. The proportion of inflammatory cells such as eosinophils, lymphocytes, plasma cells and neutrophils were calculated on the WSI of each patient through artificial intelligence chronic rhinosinusitis evaluation platform 2.0 (AICEP 2.0), and the specific type of nasal polyps was then obtained as eosinophilic CRSwNP (eCRSwNP) or non-eosinophilic CRSwNP (non-eCRSwNP). In addition, the JESREC diagnostic criteria was used to classify the nasal polyps, and the classification results were compared with the current gold standard for nasal polyps diagnosis (pathological diagnosis based on WSI). The accuracy, sensitivity and specificity of the diagnostic criteria of JESREC were evaluated. The data were expressed in M (Q1, Q3) and statistically analyzed by SPSS 17.0. Results: There was no significant difference between eCRSwNP and non-eCRSwNP in age distribution, gender, time of onset, total VAS score, Lund-Kennedy score or Lund-Mackay score. However, there was a significant difference in the ratio of nasal polyp inflammatory cells (eosinophils 40.5% (22.8%, 54.7%) vs 2.5% (1.0%, 5.3%), neutrophils 0.3% (0.1%, 0.7%) vs 1.3% (0.5%, 3.6%), lymphocytes 49.9% (39.3%, 65.9%) vs 82.0% (72.8%, 87.5%), plasma cells 5.1% (3.6%, 10.5%) vs 13.0% (7.4%, 16.3%), χ2 value was 9.91, 4.66, 8.28, 5.06, respectively, all P<0.05). In addition, eCRSwNP had a significantly higher level of proportion of allergic symptoms (nasal itching and sneezing), asthma, peripheral blood eosinophil and total IgE (all P<0.05). The overall accuracy, sensitivity and specificity of the JESREC diagnostic criteria was 74.3%, 81.3% and 64.3%, respectively. Conclusions: The eCRSwNP based on artificial intelligence and WSI has significant high level of allergic symptoms, asthma, peripheral blood eosinophils and total IgE, and the percentages of inflammatory cells in nasal polyps are different from that of non-eCRSwNP. The JESREC diagnostic criteria has good consistency in our research.


Subject(s)
Female , Humans , Male , Artificial Intelligence , Chronic Disease , Eosinophils/metabolism , Nasal Polyps/pathology , Retrospective Studies , Rhinitis/pathology , Sinusitis/pathology
2.
Arch. argent. pediatr ; 116(3): 192-197, jun. 2018. tab, graf
Article in English, Spanish | LILACS, BINACIS | ID: biblio-950009

ABSTRACT

Antecedentes. Las exacerbaciones de asma continúan siendo una causa de hospitalización en el Servicio de Urgencias. Los desencadenantesson alérgenos e infecciones, principalmente, de tipo viral. El objetivo fue determinar la relación entre los virus detectados durante la exacerbación asmática y los niveles de eosinófilos e inmunoglobulina E (IgE) sérica en pacientes pediátricos. Población y métodos. Estudio transversal analítico. Se incluyeron niños de cinco a quince años atendidos en Urgencias de Pediatría con exacerbación de asma, en el período de marzo de 2013 a febrero de 2016. Se obtuvo ácido ribonucleico viral en el aspirado nasofaríngeo con el kit CLART PneumoVir. Se cuantificaron los eosinófilos en la sangre periférica y los niveles de IgE sérica total. Se consideró eosinofilia un conteo ≥ 0,4 x 103/mm3 e IgE elevada, ≥ 350 UI/L. Se realizó la correlación de Pearson. Se definió significancia con valor de p ≤ 0,05.Resultados. De 211 niños con exacerbación de asma, en el 20%, se aisló un virus. Los virus aislados más frecuentemente fueron el rinovirus, el enterovirus y el virus sincitial respiratorio. Se encontró una correlación entre los niveles de eosinófilos e IgE sérica total en los niños con exacerbación de asma y rinovirus de 0,89, con una p= 0,0001.Conclusiones. Las infecciones por rinovirus, enterovirus y virus sincitial respiratorio son más frecuentes en las exacerbaciones de asma en menores de 15 años. Se observó una correlación entre los niveles de eosinófilos e IgE en presencia de rinovirus.


Background. Asthma exacerbations are still a cause of hospitalization at the Emergency Department. The triggers of asthma exacerbations include allergens and infections ­mostly viral­. The objective of this study was to establish the relationship between viruses detected during an asthma exacerbation and eosinophil and serum immunoglobulin E (IgE) levels in pediatric patients. Population and methods. Cross-sectional. analytical study. Children aged 5-15 years seen at the Pediatric Emergency Department with an asthma exacerbation in the period between March 2013 and February 2016 were included. Viral ribonucleic acid was extracted from nasopharyngeal aspirates using the CLART Pneumo Vir kit. Eosinophil levels were measured in peripheral blood and total IgE levels, in serum. Eosinophilia was defined as a count ≥ 0.4 x 103/mm3 and high IgE. as a level ≥ 350 IU/L. The Pearson's correlation was carried out. A value of p ≤ 0.05 was considered significant.Results. Out of 211 children with asthma exacerbation, a virus was isolated in 20%. The most commonly isolated viruses were rhinovirus. enterovirus, and respiratory syncytial virus. A correlation of 0.89 was established between eosinophil and total serum IgE levels in children with asthma exacerbation and rhinovirus, with a p value of 0.0001. Conclusions. Rhinovirus, enterovirus, and respiratory syncytial virus were the most common viruses in asthma exacerbations in children younger than 15 years. A correlation was established between eosinophil and IgE levels in the presence of rhinovirus.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Asthma/virology , Immunoglobulin E/blood , Eosinophils/metabolism , Asthma/physiopathology , Asthma/blood , Rhinovirus/isolation & purification , Cross-Sectional Studies , Respiratory Syncytial Virus, Human/isolation & purification , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Virus Infections/epidemiology , Enterovirus/isolation & purification , Picornaviridae Infections/diagnosis , Picornaviridae Infections/epidemiology , Emergency Service, Hospital , Enterovirus Infections/diagnosis , Enterovirus Infections/epidemiology
3.
Einstein (Säo Paulo) ; 13(4): 560-566, Oct.-Dec. 2015. tab, graf
Article in Portuguese | LILACS | ID: lil-770487

ABSTRACT

ABSTRACT Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.


RESUMO Objetivo Desenvolver um novo modelo experimental de doença pulmonar alérgica crônica por ácaro, com proeminente produção de imunoglobulina E (IgE) específica, infiltrado inflamatório eosinofílico nas vias aéreas e remodelamento, comparando duas vias diferentes de sensibilização. Métodos O protocolo teve duração de 30 dias. Camundongos BALB/c foram divididos em seis grupos submetidos à sensibilização por via subcutânea ou intraperitoneal com solução salina (controles negativos),Dermatophagoides pteronyssinus (Der p) 50 ou 500mcg, em três aplicações. Posteriormente, foram submetidos à provocação intranasal com Der p ou salina por 7 dias e sacrificados 24 horas após o último desafio. Avaliamos a titulação de IgE específica anti-Der p, densidade eosinofílica no espaço peribroncovascular e remodelamento das vias aéreas. Resultados Tanto os animais sensibilizados por via subcutânea como intraperitoneal produziram IgE específica anti-Der p. Ocorreu aumento da eosinofilia peribroncovascular apenas nos animais que receberam menor dose de Der p. Porém apenas o grupo sensibilizado com Der p 50mcg subcutânea apresentou remodelamento significativo das vias aéreas. Conclusão Neste modelo murino de asma, as duas vias de sensibilização levaram à produção de IgE específica e eosinofilia nas vias aéreas. No entanto, apenas a via subcutânea foi capaz de induzir ao remodelamento. Além disso, doses menores de Der p utilizadas foram superiores às mais elevadas, sugerindo tolerância. Mais estudos são necessários para avaliar a eficácia deste modelo no desenvolvimento da hiperresponsividade brônquica, mas ele pode ser replicado em experimentos para criação de novas estratégias terapêuticas medicamentosas ou imunoterápicas.


Subject(s)
Animals , Male , Allergens/administration & dosage , Asthma/immunology , Disease Models, Animal , Immunization/methods , Pyroglyphidae , Administration, Intranasal , Asthma/physiopathology , Bronchial Provocation Tests , Enzyme-Linked Immunosorbent Assay , Eosinophils/metabolism , Fibrillar Collagens/metabolism , Injections, Intraperitoneal , Injections, Subcutaneous , Immunoglobulin E/blood , Immunoglobulin G/blood , Leukocyte Count , Mice, Inbred BALB C , Passive Cutaneous Anaphylaxis/immunology , Pulmonary Eosinophilia/parasitology
4.
Rev. chil. pediatr ; 86(3): 206-213, jun. 2015. tab
Article in Spanish | LILACS | ID: lil-760116

ABSTRACT

La evaluación del control del asma considera la medición de la sintomatología, la calidad de vida, la función pulmonar y los marcadores inflamatorios. En los últimos años se han multiplicado las publicaciones relacionadas con el estudio de biomarcadores en el manejo del asma pediátrica. A pesar de la gran variedad de marcadores inflamatorios descritos en estudios de investigación, solo un pequeño grupo ha demostrado ser útil en el control de la enfermedad. Los eosinófilos en esputo inducido tienen la evidencia más sólida en la medición del control de asma. El condensado de aire exhalado y los leucotrienos urinarios podrían tener utilidad en el futuro si se logran estandarizar sus procedimientos e interpretación de resultados. El óxido nítrico, la proteína catiónica básica del eosinófilo y la biopsia bronquial con lavado broncoalveolar parecieran tener utilidad solo en un grupo reducido de pacientes.


The assessment of asthma control takes into account the symptoms, quality of life, lung function, and inflammatory markers. In the last few years, there has been a large increase in the number of publications related to the study of biomarkers in the management of paediatric asthma. Despite the large variety of inflammatory markers described in research studies, only a small group has shown to be useful in monitoring the disease. Induced sputum eosinophils offer the most solid evidence in assessing asthma control. Exhaled breath condensate and urinary leucotrienes could be useful in the future if there is standardisation in their procedures and interpretation of the results. Nitric oxide, basic eosinophil cationic protein, and bronchial biopsy with bronchoalveolar lavage, only appeared to be useful in a reduced group of patients.


Subject(s)
Humans , Child , Asthma/physiopathology , Biomarkers/metabolism , Inflammation/physiopathology , Quality of Life , Asthma/diagnosis , Asthma/therapy , Sputum , Bronchoalveolar Lavage/methods , Eosinophils/metabolism , Nitric Oxide/metabolism
5.
Int. j. odontostomatol. (Print) ; 9(1): 91-95, Apr. 2015. ilus
Article in English | LILACS | ID: lil-747483

ABSTRACT

Tumor related tissue eosinophilia (TATE) is a known phenomenon but its role in prognostics and correlation with size of the primary tumor is still controversial. Using a stain, like Carbol chromotrope, that targets eosinophils exclusively and vividly, offers an advantage over haematoxylin and eosin, which was used in most of the studies. Forty-nine cases of oral squamous cell carcinoma, where the TNM staging has been recorded in their history, was taken and stained with Lendrum's carbol chromotrope. Significant difference in the eosinophil count with varying size of the tumor and a parallel increase in the number noted, with increase in size. There is a corresponding increase in the number of eosinophils infiltrating the tumor with increase in size of oral squamous cell carcinoma.


La eosinofilia tisular asociada a tumores (TATE) es un fenómeno conocido, sin embargo su pronóstico y correlación con el tamaño del tumor primario aún es tema de controversia. El uso de cromotropo como tinción dirigida exclusivamente a los eosinófilos, ofrece una ventaja sobre la hematoxilina-eosina, que ha sido utilizada en la mayoría de los estudios. Se estudiaron células escamosas en 49 casos de carcinoma oral, con registro del estadio TNM. Las células fueron teñidas con carbol cromotropo de Lendrum. Se observó una diferencia significativa en el recuento de eosinófilos con el tamaño del tumor y un aumento paralelo en número, con el aumento de tamaño. Hay un aumento correspondiente en el número de eosinófilos que infiltran el tumor con aumento en el tamaño de carcinoma de células escamosas orales.


Subject(s)
Humans , Mouth Neoplasms/complications , Carcinoma, Squamous Cell/complications , Eosinophilia/complications , Eosinophils/metabolism , Squamous Cell Carcinoma of Head and Neck/complications , Prognosis , Cell Differentiation , Analysis of Variance , Color Therapy , Coloring Agents , Eosinophils/pathology , Tumor Microenvironment , Neoplasm Invasiveness
6.
J. pediatr. (Rio J.) ; 89(2): 197-203, mar.-abr. 2013. tab
Article in Portuguese | LILACS | ID: lil-671456

ABSTRACT

OBJECTIVE: This study aimed to describe the clinical, endoscopic, and histologic characteristics, as well as the response to conventional treatment of pediatric patients with the classical form of eosinophilic esophagitis (EoE). METHODS: Study of clinical, laboratory, endoscopic, and histologic data and response to conventional treatment of 43 previously followed pediatric patients with the classical form of EoE. RESULTS: A total of 43 patients diagnosed with EoE were included in the study, of which 37 were males (86%), with a mean age of 8.4 years. The most common symptoms were: nausea, vomiting, and abdominal pain (100%) in children younger than 7 years, and loss of appetite (60%), heartburn (52%), and food impaction (48%) in children older than 7 years and adolescents. Regarding the endoscopic findings, 12 (28%) patients had whitish plaques on the esophageal lining, 8 (18.5%) had longitudinal grooves, 2 (4.5%) had concentric rings, 3 (7%) had longitudinal grooves and whitish plaques, and the remaining 18 (42%) had esophageal mucosa with normal appearance. Despite the initial favorable response, 76.7% of patients required more than one course of corticosteroid therapy (systemic or aerosol) and diet (exclusion or elimination of food or elementary allergens). Persistence of eosinophil infiltration was found in some patients despite favorable clinical response. CONCLUSIONS: The classic form of EoE typically shows different symptoms according age range. A significant number of patients required more than one treatment cycle to show clinical remission. Endoscopic and histologic improvement was observed; however, eosinophilic infiltration persisted in some patients.


OBJETIVO: O objetivo deste estudo foi descrever as características clínicas, endoscópicas e histológicas, assim como a resposta ao tratamento convencional de pacientes pediátricos com a forma clássica de esofagite eosinofílica (EEo). MÉTODOS: Levantamento de dados clínicos, laboratoriais, endoscópicos, histológicos e da resposta ao tratamento convencional de 43 pacientes pediátricos acompanhados previamente com a forma clássica de EEo. RESULTADOS: Foram incluídos 43 pacientes com diagnóstico de EEo, sendo 37 do sexo masculino (86%), com idade média de 8,4 anos. Os sintomas mais encontrados foram: náusea, vômito e dor abdominal (100%) em crianças menores de sete anos; e inapetência (60%), queimação retroesternal (52%) e impactação alimentar (48%) em crianças maiores de sete anos e adolescentes. Em relação aos achados endoscópicos, 12 (28%) pacientes apresentavam placas esbranquiçadas na mucosa do esôfago, oito (18,5%) sulcos longitudinais, dois (4,5%) anéis concêntricos, três (7%) sulcos longitudinais e placas esbranquiçadas, e os outros 18 (42%) apresentavam aparência normal da mucosa esofágica. Apesar da resposta favorável inicial, 76,7% dos pacientes necessitaram realizar mais de um ciclo terapêutico com corticoterapia (aerossol ou sistêmica) e dieta (de exclusão ou eliminação dos alérgenos alimentares ou elementares). Persistência do infiltrado eosinofílico foi encontrada em uma parcela dos pacientes, a despeito da resposta clínica favorável. CONCLUSÕES: A forma clássica da EEo apresenta sintomas diferentes segundo a faixa etária. Parcela expressiva dos pacientes necessitou de mais de um ciclo terapêutico para apresentar remissão clínica. Observou-se melhora endoscópica e histológica; no entanto, a infiltração eosinofílica persistiu em parcela dos pacientes.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Infant , Male , Eosinophilic Esophagitis , Chi-Square Distribution , Endoscopy, Digestive System , Eosinophilic Esophagitis/complications , Eosinophilic Esophagitis/diet therapy , Eosinophilic Esophagitis/metabolism , Eosinophilic Esophagitis/pathology , Eosinophils/metabolism , Glucocorticoids/therapeutic use , Immunoglobulin E/blood , Retrospective Studies , Treatment Outcome
7.
The Korean Journal of Parasitology ; : 373-380, 2011.
Article in English | WPRIM | ID: wpr-78169

ABSTRACT

We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-alpha (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.


Subject(s)
Animals , Female , Mice , Administration, Intranasal , Anisakiasis/immunology , Anisakis/immunology , Bronchoalveolar Lavage Fluid , Chemokines/metabolism , Cytokines/analysis , Eosinophils/metabolism , Gene Expression Regulation/immunology , Helminth Proteins/immunology , Hypersensitivity/immunology , Immunoglobulin E/immunology , Immunoglobulin G/immunology , Larva/immunology , Lung/metabolism , Mice, Inbred C57BL , Recombinant Proteins/immunology , Th17 Cells/metabolism , Th2 Cells/metabolism
8.
Yonsei Medical Journal ; : 744-750, 2009.
Article in English | WPRIM | ID: wpr-43538

ABSTRACT

Aspirin intolerant asthma (AIA) is frequently characterized as an aspirin (ASA)-exacerbated respiratory disease (AERD). It is a clinical syndrome associated with chronic severe inflammation in the upper and lower airways resulting in chronic rhinitis, sinusitis, recurrent polyposis, and asthma. AERD generally develops secondary to abnormalities in inflammatory mediators and arachidonic acid biosynthesis expression. Upper and lower airway eosinophil infiltration is a key feature of AERD; however, the exact mechanisms of such chronic eosinophilic inflammation are not fully understood. Cysteinyl leukotriene over-production may be a key factor in the induction of eosinophilic activation. Genetic studies have suggested a role for variability of genes in disease susceptibility and response to medication. Potential genetic biomarkers contributing to the AERD phenotype include HLA-DPB1* 301, LTC4S, ALOX5, CYSLT, PGE2, TBXA2R, TBX21, MS4A2, IL10 -1082A > G, ACE -262A > T, and CRTH2 -466T > C; the four-locus SNP set was composed of B2ADR 46A > G, CCR3 -520T > G, CysLTR1 -634C > T, and FCER1B -109T > C. Management of AERD is an important issue. Aspirin ingestion may result in significant morbidity and mortality, and patients must be advised regarding aspirin risk. Leukotriene receptor antagonists (LTRA) that inhibit leukotriene pathways have an established role in long-term AERD management and rhinosinusitis. Aspirin desensitization may be required for the relief of upper and lower airway symptoms in AERD patients. Future research should focus on identification of biomarkers for a comprehensive diagnostic approach.


Subject(s)
Animals , Humans , Asthma, Aspirin-Induced/drug therapy , Eosinophils/metabolism , Genetic Predisposition to Disease/genetics , Leukotriene Antagonists/therapeutic use , Leukotrienes/metabolism , Models, Biological , Polymorphism, Genetic/genetics
9.
Journal of Korean Medical Science ; : 903-905, 2008.
Article in English | WPRIM | ID: wpr-168521

ABSTRACT

Albendazole binds to parasite's tubulin inhibiting its glucose absorption. Its common adverse effects are nausea, vomiting, constipation, thirst, dizziness, headache, hair loss and pruritus. Although mainly metabolized in the liver, abnormal liver function tests were a rare adverse effect during clinical trials and we found no literature about albendazole-induced hepatitis requiring admission. This patient had a previous history of albendazole ingestion in 2002 resulting in increase of liver function tests. And in 2005, the episode repeated. We evaluated the patient for viral hepatitis, alcoholic liver disease, and autoimmune hepatitis, but no other cause of hepatic injury could be found. Liver biopsy showed periportal steatosis and periportal necrosis. The initial abnormal liver function test improved only with supportive care. These findings and the Roussel Uclaf Causality Assessment Method of the Council for International Organizations of Medical Sciences (RUCAM/CIOMS) score of 9 are compatible with drug-induced hepatitis so we report the case of this patient with a review of the literature.


Subject(s)
Humans , Male , Middle Aged , Albendazole/adverse effects , Antiprotozoal Agents/adverse effects , Eosinophils/metabolism , Glucose/metabolism , Chemical and Drug Induced Liver Injury/diagnosis , Liver/drug effects , Liver Function Tests , Necrosis
11.
Journal of Korean Medical Science ; : 815-819, 2007.
Article in English | WPRIM | ID: wpr-176606

ABSTRACT

The house dust mite (HDM) is considered to be the most common indoor allergen associated with bronchial asthma. In this study, we investigated whether crude extract of the HDM Dermatophagoides farinae could activate human eosinophilic leukemic cells (EoL-1) to induce upregulation of cell-surface adhesion molecules. When EoL-1 cells were incubated with D. farinae extract, expression of intercellular adhesion molecule-1 (ICAM-1) significantly increased on the cell surfaces compared to cells incubated with medium alone. In contrast, surface expression of CD11b and CD49d in EoL-1 cells was not affected by D. farinae extract. In addition, pretreatment of cells with NF- kappaB inhibitor (MG-132) or JNK inhibitor (SP600125) significantly inhibited ICAM-1 expression promoted by HDM extract. However, neither p38 MAP kinase inhibitor nor MEK inhibitor prevented HDM-induced ICAM-1 expression in EoL-1 cells. These results suggest that crude extract of D. farinae induces ICAM-1 expression in EoL-1 cells through signaling pathways involving both NF- kappaB and JNK.


Subject(s)
Animals , Humans , Anthracenes/pharmacology , CD11b Antigen/biosynthesis , Cell Line, Tumor , Cell Membrane/metabolism , Eosinophils/metabolism , Flow Cytometry/methods , Gene Expression Regulation , Integrin alpha4/biosynthesis , Intercellular Adhesion Molecule-1/metabolism , Leukemia/metabolism , Leupeptins/pharmacology , Mitogen-Activated Protein Kinase 8/metabolism , NF-kappa B/metabolism , Pyroglyphidae , p38 Mitogen-Activated Protein Kinases/metabolism
12.
Rev. bras. otorrinolaringol ; 72(1): 38-42, jan.-fev. 2006. ilus, tab, graf
Article in Portuguese, English | LILACS | ID: lil-434978

ABSTRACT

A polipose nasossinusal eosinofílica (PNS) é manifestação de uma doença inflamatória crônica na mucosa do nariz e nos seios paranasais caracterizada por infiltração de granulócitos eosinófilos. O fator responsável pela eosinofilia e manutenção dessas células com a perpetuação do processo inflamatório e formação polipóide é objeto constante de estudos. As citocinas como IL5 (interleucina 5) e GM-CSF (fator estimulador de colônia granulócito macrófago) aumentam a sobrevida dos eosinófilos e prolongam a sua presença no tecido polipóide, diminuindo o índice de apoptose eosinofílica. OBJETIVO: Avaliar o efeito da mitomicina C - MMC - por meio de aplicação tópica em pacientes portadores de PNS eosinofílica quanto à presença de IL5 e GM-CSF. CASUÍSTICA E MÉTODOS: Quinze pacientes portadores de PNS eosinofílica foram submetidos à aplicação tópica de MMC na concentração de 0,5mg/ml, 1ml, durante cinco minutos, na cavidade nasal direita, e submetidos à biópsia para RT-PCR 24hs após. O grupo-controle foi a cavidade nasal esquerda. O perfil de citocinas foi analisado para IL5 e GM-CSF. RESULTADOS: A comparação dos resultados de GM-CSF pré e pós-uso de MMC quando usamos o teste t pareado apresenta p=0,041. A comparação para IL5 resulta em p < 0,001. CONCLUSÃO: O uso de MMC em pacientes com PNS mostra redução com significância estatística par GM-CSF e importante significância para IL5.


Eosinophilic nasosinusal polyposis is a chronic inflammatory infection with elevated infiltration of eosinophils, which presents high rate of recurrence after surgical treatment. The continuous inflammatory process that leads to the formation of polyps requires constant clinical treatment. Contributing to the maintenance of eosinophilia are cytokines IL5 (interleukin-5) and GM-CSF (granulocyte macrophages colony-stimulating factor), which show up in elevated concentrations. These oligoproteins diminish the rate of apoptosis and prolong the survival of eosinophils. AIM: By diminishing these cytokines, the action of Mitomycin C (MMC), an antineoplasic drug which inhibits the synthesis of DNA, was studied. In a recent study the power of this drug to cause apoptosis in eosinophils, in vitro, of nasal polyps was verified. METHODOLOGY: A biopsy of the nasal polyps was undertaken in 15 patients carriers of eosinophilic nasosinusal polyposis 24 hours after applying 0.5 mg/ml of MMC during five minutes. RT-PCR (reverse transcription of polymerase chain reaction) for IL5 and GM-CSF was the method used to obtain the results. RESULTS: The comparison of the results of GM-CSF pre- and post-application of MMC, when the paired T-test was used, showed p=0.041 and for IL5 we found p<0.001. CONCLUSION: Topic use of MMC in patients with eosinophilic nasosinusal polyposis shows statistically significant reduction for GM-CSF and significant and important reduction for IL5.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Eosinophilia/drug therapy , Granulocyte-Macrophage Colony-Stimulating Factor , Nucleic Acid Synthesis Inhibitors/therapeutic use , /metabolism , Mitomycin/therapeutic use , Nasal Polyps/drug therapy , Eosinophilia/metabolism , Eosinophils/drug effects , Eosinophils/metabolism , Granulocyte-Macrophage Colony-Stimulating Factor , Nasal Polyps/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Time Factors
13.
Medicina (B.Aires) ; 66(5): 405-414, 2006. tab, graf, ilus
Article in Spanish | LILACS | ID: lil-451707

ABSTRACT

La neutropenia inmune se diagnostica por la presencia de auto o aloanticuerpos reactivos con losneutrófilos. La neutropenia aloinmune neonatal es consecuencia de la sensibilización materna alos antígenos específicos de los neutrófilos paternos que afectan al neonato al atravesar la barrera placentaria. Se presentan 4 casos de niños, 2 de ellos hermanos consanguíneos con doble vínculo. Se estudiaron los sueros de los pacientes y sus padres. Por citometría de flujo se establecen los valores de referencia de la IgG sérica reactiva con los neutrófilos en voluntarios sanos, para 3 diluciones (1/2, 1/5 y 1/20) en reacción autóloga(suero y células de un mismo individuo) y heteróloga (suero y células de diferentes individuos). Los resultadosse expresan por un índice definido como el cociente entre la mediana de la intensidad de fluorescencia media del suero incógnita y la de un suero utilizado como referencia. Por leucoaglutinación se evaluó la dilucióndel suero 1/20. Se determinó el nivel de complejos inmunes circulantes. Se determinó el fenotipo, para los epitopes HNA-1a, HNA-1b y HNA-2a. En los 4 niños se encontró IgG reactiva y/o factores aglutinantes; 2/3 sueros maternos fueron reactivos con los neutrófilos del cónyuge y de los hijos. Los complejos inmunes circulantes fueron positivos en 2/4 sueros negativos en 3/3 sueros maternos. Se encontró incompatibilidad materno-infantil en los 4 casos. Las 3 madres tenían igual fenotipo: homocigotos NA1/NA1, NB1+. En síntesis, se presenta el hallazgo de 4 casos con neutropenia inmune: 3/4 auto-inmune, 1/3 se asocia a complejos inmunes circulantes y 1/4 con neutropenia neonatal aloinmune


Auto or alloantibodies reactive with neutrophils define immune neutropenia. Alloimmune neonatal neutropenia is caused by maternal sensitization to paternal neutrophil antigens, resulting in IgG antibodies that are transferred to the fetus through the placenta. We present the studies in 4 children from 3 families with neutropenia of unknown origin (two of them were brothers). Theywere evaluated by flow cytometry in parallel with leukoagglutination. Reference values were established forserum reactive IgG in healthy volunteers for three dilutions (1/2, 1/5 and 1/20), both for the autologous reaction (serum and cells of the same individual) and for the heterologous reaction (serum and cells of differentindividuals). Results were expressed by an index defined by the quotient of the mean fluorescence intensityof the patient’s serum divided by that of the reference serum. Serum reactive/agglutinant factors and circulating immune complexes were evaluated in patients and parents serum. Neutrophil specific phenotypes weredetermined for HNA-1a, HNA-1b and HNA-2a. Reactive IgG/agglutinant factors were found in 4 children. Twomaternal sera were reactive against paternal and/or children neutrophils. Circulating immune complexes weredetected in 2/4 children sera and were negative in 3/3 maternal sera. Maternal/children incompatibility wasdetected in the four cases. The three mothers had the same phenotype: homozygous NA1/NA1, NB1+


Subject(s)
Humans , Male , Female , Pregnancy , Infant , Antibodies, Antineutrophil Cytoplasmic/immunology , Flow Cytometry/methods , Immunoglobulin G/blood , Neutropenia/immunology , Neutrophils/immunology , Agglutination/immunology , Antibodies, Antineutrophil Cytoplasmic/blood , Eosinophils/metabolism , Leukocyte Count , Neutropenia/blood , Neutrophils/metabolism , Phenotype , Reference Values
14.
Journal of Medical Council of Islamic Republic of Iran. 2006; 24 (2): 113-118
in Persian | IMEMR | ID: emr-77966

ABSTRACT

Allergic rhinitis is a chronic disease of upper respiratory tract which can be presented as asthma. Some investigators have reported a significant relationship between the disease and the number of eosinophils in nasal secretion of the patients. This study aims to evaluate the serum IgE and it's relation with nasal secretion eosinophils count in patients with allergic rhinitis. In this study, serum IgE of 92 patients with non anatomical nasal complication were investigated using ELISA method [IEMA WELL Q-D]. Nasal secretion smears of the patients were also evaluated for eosinophils count. Age, sex, profession and past history of allergic reactions in patients and their families were also investigated. 48. 9% of the patients had high titers of serum IgE [>100 IU/ml]. Also, eosinophils over 5/HPF were seen in 15. 6% of patients, nasal secretion smears. There was no significant relationship between eosinophils count and presence of allergic rhinitis in the patients. Otherwise, there was a significant correlation between rhinitis and patients, profession and their locality of living [P<0. 05]. Detection of serum IgE is mandatory for diagnosis of allergic rhinitis. Besides, it seems that nasal secretion eosinophils count alone is not a reliable finding for diagnosis of the disease


Subject(s)
Female , Humans , Male , Rhinitis, Allergic, Seasonal/diagnosis , Rhinitis, Allergic, Seasonal/blood , Enzyme-Linked Immunosorbent Assay , /blood , Eosinophils/metabolism
15.
Rev. bras. otorrinolaringol ; 71(4): 459-463, jul.-ago. 2005. graf
Article in Portuguese | LILACS | ID: lil-419328

ABSTRACT

O estudo de fatores teciduais, como a concentração de fator estimulador de colônias de macrófagos (GM-CSF) e interleucina 5 (IL-5), aponta para os mecanismos envolvidos na manutenção da eosinofilia em pólipos nasossinusais eosinofílicos. A mitomicina C (MMC) tem sido utilizada com bons resultados em otorrinolaringologia. OBJETIVO: Este estudo teve como objetivo avaliar a ação da Mitomicina C sobre a secreção de GM-CSF e IL-5 em pólipos eosinofílicos. FORMA DE ESTUDO: caso-controle. MATERIAL E MÉTODO: O estudo foi comparativo experimental autopareado, com amostras de pólipos biopsiados de pacientes portadores de polipose nasossinusal eosinofílica. Os fragmentos semeados como grupo experimental receberam mitomicina C por 5 minutos na dosagem de 400microg/ml e então lavadas em meio RPMI. Nos tempos zero, 12 e 24 horas, o sobrenadante foi retirado para determinação dos níveis de GM-CSF em 22 pacientes e IL-5, em 19 pacientes, utilizando o método de ELISA. RESULTO: Diminuição de secreção de GM-CSF nos grupos tratados com mitomicina C no tempo 24h (p<= 0,05); no grupo tratado houve expressão significativa de GM-CSF entre zero e 12 horas (p=0,013) demonstrando a viabilidade da cultura igualmente ao grupo não tratado; tendência à queda dos níveis de IL-5 no grupo tratado em 24h. CONCLUSÃO: O estudo demonstrou que a mitomicina C foi capaz de inibir a síntese de GM-CSF em culturas de pólipos nasais eosinofílicos e com provável ação sobre a secreção de IL-5, necessitando de estudos complementares.


Subject(s)
Humans , Adolescent , Adult , Middle Aged , Eosinophils/drug effects , Granulocyte-Macrophage Colony-Stimulating Factor , Nucleic Acid Synthesis Inhibitors/pharmacology , /biosynthesis , Mitomycin/pharmacology , Nasal Polyps/metabolism , Biopsy , Case-Control Studies , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Eosinophils/metabolism , Nasal Polyps/pathology , Time Factors
16.
Journal of Korean Medical Science ; : 489-493, 2003.
Article in English | WPRIM | ID: wpr-156014

ABSTRACT

The monitoring of airway inflammation has assessed in bronchial asthma directly by sputum examination, and indirectly by measurements in peripheral blood. To investigate the diagnostic value of these two methods, we compared nitric oxide (NO) metabolites, eosinophils, and eosinophil cationic protein (ECP) in sputum and blood in patients with asthma and control subjects. Sputum and serum were obtained from fifteen patients with asthma, and then were examined before anti-asthma treatment, including steroid preparations. ECP was measured by fluoroimmunoassay. NO metabolites were assayed by using modified Griess reaction. Asthmatic patients, compared with control subjects, had significantly higher level of NO metabolites, higher proportion of eosinophils, and higher levels of ECP in sputum. Asthmatic patients, compared with control subjects, however, had significantly higher number of eosinophils, and were at higher levels of ECP in blood. FEV1, FEV1 /FVC was negatively correlated with sputum eosinophils. The area under receiver operating characteristic(ROC) curve showed that eosinophils in sputum are significantly accurate markers than NO metabolites in sputum and blood. These findings suggest that the proportion of eosinophils in sputum have more accurate diagnostic marker of asthmatic airway inflammation than NO metabolites in sputum in differentiating asthmatic patients from control subjects.


Subject(s)
Adult , Female , Humans , Male , Area Under Curve , Asthma/blood , Blood Proteins/metabolism , Comparative Study , Eosinophils/metabolism , Fluoroimmunoassay , Inflammation , Nitrates/metabolism , Nitric Oxide/blood , Nitrites/metabolism , ROC Curve , Ribonucleases/blood , Sputum/metabolism
17.
Journal of Korean Medical Science ; : 833-839, 2002.
Article in English | WPRIM | ID: wpr-125135

ABSTRACT

Hematopoietic neoplasm coexpressing CD4 and CD56 includes a subset of acute myeloid leukemia with myelomonocytic differentiation, plasmacytoid monocyte tumor, and other immature hematopoietic neoplasms of undefined origin. Herein, we report a CD4+CD56+CD68+ hematopoietic tumor that was thought to be a tumor of plasmacytoid monocytes. This case is unique in the absence of accompanying myelomonocytic leukemia and the faint expression of cCD3 on the tumor cells. The patient was a 22-yr old man presented with multiple lymphadenopathy and an involvement of the bone marrow. Tumor cells were large and monomorphic with an angulated eosinophilic cytoplasm of moderate amount. Nuclei of most tumor cells were eccentric and round with one or two prominent nucleoli. Rough endoplasmic reticulum was prominent in electron microscopic examination. Tumor cells expressed CD4, CD7, CD10, CD45RB, CD56, CD68, and HLA-DR and were negative for CD1a, CD2, sCD3, CD5, CD13, CD14, CD20, CD33, CD34, CD43, CD45RA, TIA-1, S-100, and TdT. cCD3 was not detected in the immunostaining using paraffin tissue, but was faintly expressed in flow cytometry and immunostaining using a touch imprint slide. T-cell receptor gene rearrangement analysis and EBV in situ hybridization showed negative results. Cytochemically, myeloperoxidase, Sudan black B, and alpha naphthyl butyrate esterase were all negative.


Subject(s)
Adult , Humans , Male , Antigens, CD/biosynthesis , CD3 Complex/biosynthesis , CD4 Antigens/biosynthesis , Leukocyte Common Antigens/biosynthesis , CD56 Antigen/biosynthesis , Antigens, Differentiation, Myelomonocytic/biosynthesis , Bone Marrow Cells/pathology , Cell Nucleus/pathology , Eosinophils/metabolism , Flow Cytometry , Gene Rearrangement , Immunohistochemistry , In Situ Hybridization , Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis , Lymph Nodes/pathology , Microscopy, Electron , Monocytes/metabolism , Receptors, Antigen, T-Cell/metabolism
18.
Journal of Korean Medical Science ; : 521-528, 2000.
Article in English | WPRIM | ID: wpr-150740

ABSTRACT

Corticosteroids are considered to be one of the most effective medicine for asthma by suppressing airway inflammation. This study was carried out to investigate the effects of prednisolone in the sputum of exacerbated asthmatics. Clinical severity, cell differentials, levels of interleukin (IL)-5, eosinophil cationic protein (ECP), EG2+ eosinophils, and nitric oxide (NO) metabolites were measured. Sputum was examined 2 weeks apart in 13 exacerbated asthmatics before and after prednisolone treatment, and once in 12 stable asthmatics. We used a sandwich ELISA for IL-5, fluoroimmunoassay for ECP, immunohistochemical staining for EG2+ eosinophils, a NO metabolites assay using modified Griess reaction. Exacerbated asthmatics, in comparison with stable asthmatics, had significantly higher proportion of eosinophils, higher level of ECP, higher percentage of EG2+ eosinophils, and NO metabolites. Exacerbated asthmatics after treatment with prednisolone had reduced the proportions of eosinophils, reduced level of IL-5, ECP and percentage of EG2+ eosinophils. FEV1 was correlated with the proportion of eosinophils, ECP, and IL-5 respectively. These findings suggest that prednisolone is considered to be effective medicine for asthma by suppressing eosinophil activation through IL-5.


Subject(s)
Adult , Aged , Female , Humans , Male , Administration, Oral , Adolescent , Adrenal Cortex/metabolism , Anti-Inflammatory Agents/administration & dosage , Asthma/metabolism , Asthma/immunology , Asthma/drug therapy , Biomarkers , Blood Proteins/metabolism , Eosinophils/metabolism , Eosinophils/immunology , Eosinophils/drug effects , Interleukin-5/metabolism , Leukocyte Count , Middle Aged , Nitric Oxide/metabolism , Prednisolone/administration & dosage , Sputum/immunology , Sputum/cytology
19.
Arch. argent. alerg. inmunol. clín ; 21(4): 130-5, dic. 1990. ilus
Article in Spanish | LILACS | ID: lil-100745

ABSTRACT

Se presenta un caso de una paciente de 49 años de edad, sexo femenino, con diagnóstico de neumonía eosinifílica crónica asociada con asma. Se describen las características clínicas, radiológicas y de laboratorio. Presenta antecedentes familiares alérgicos, una historia aprevia de rinitis perenne y asma bronquial de larga evolución. El diagnóstico de este paciente se realizó teniendo en cuenta: 1) el cuadro clínico; 2) la hipereosinofilia periférica, y 3) el infiltrado pulmonar característico. Además se consideró la respuesta espectacular al tratamiento corticoideo, con desaparición de los síntomas clínicos y radiológicos con cinco días de tratamiento


Subject(s)
Middle Aged , Female , Humans , Asthma/complications , Eosinophilia/etiology , Pulmonary Eosinophilia/etiology , Adrenal Cortex Hormones/therapeutic use , Aspergillosis, Allergic Bronchopulmonary/diagnosis , Beclomethasone/therapeutic use , Chronic Disease , Diagnosis, Differential , Eosinophils/immunology , Eosinophils/metabolism , Eosinophilia/classification , Pulmonary Eosinophilia , Pulmonary Eosinophilia/drug therapy , Rhinitis, Allergic, Perennial/complications , Tuberculosis, Pulmonary/diagnosis
20.
Arch. argent. dermatol ; 39(2): 73-88, mar.-abr. 1989. tab
Article in Spanish | LILACS | ID: lil-95802

ABSTRACT

Se efectúa una revisión crítica de 46 casos del síndrome de Wells (Celulitis eosinofílica) precisándose las características clínicas, histopatológicas, inmunológicas, etiopatogénicas y terapéuticas. En la paciente aquí descripta, la asociación de cimetidina con corticoides produjo una involución total de las lesiones.


Subject(s)
Humans , Middle Aged , Female , Cellulitis/physiopathology , Eosinophilic Granuloma/pathology , Adrenal Cortex Hormones/therapeutic use , Blister , Cimetidine/therapeutic use , Edema , Eosinophils/metabolism , Eosinophilia/pathology , Erythema , Foot Dermatoses/pathology , Eosinophilic Granuloma/epidemiology , Eosinophilic Granuloma/drug therapy , Leg Dermatoses/pathology
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